The Bucher Lab

Model Plant and Crop Epigenetics

Inhibition of RNA polymerase II allows controlled mobilisation of retrotransposons for plant breeding.  

M. Thieme, S. Lanciano, S. Balzergue, N. Daccord, M. Mirouze and E. Bucher.

Genome Biol (2017) 18, 134.

High-quality de novo assembly of the apple genome and methylome dynamics of early fruit development.  

N. Daccord, J. M. Celton, G. Linsmith, C. Becker, N. Choisne, E. Schijlen, H. van de Geest, L. Bianco, D. Micheletti, R. Velasco, E. A. Di Pierro, J. Gouzy, D J. G Rees, P. Guérif, H. Muranty, C. E. Durel, F. Laurens, Y. Lespinasse, S. Gaillard, S. Aubourg, H. Quesneville, D. Weigel, E. Van de Weg, M. Troggio, and E. Bucher.

Nat Genet (2017) 49, 1099–1106.

Recurrent evolution of heat-responsiveness in Brassicaceae COPIA elements. 

B. Pietzenuk, C. Markus, H. Gaubert, N. Bagwan, A. Merotto, E. Bucher, and A. Pecinka.

Genome Biol (2016) vol. 17 (1) p. 209.

Heterosis and inbreeding depression of epigenetic Arabidopsis hybrids. 

M. Dapp, J. Reinders, A. Bédiée, C. Balsera, E. Bucher, G. Theiler, C. Granier and J. Paszkowski

Nature Plants (2015)

HDA6 controls gene expression patterning and DNA methylation-independent euchromatic silencing.

E. Hristova, K. Fal, L. Klemme, D. Windels and E. Bucher

Plant Physiology (2015) pp.00177.2015

The return of Lamarck? 

E.Bucher

Frontiers in Genetics (2013), vol. 4

 

Epigenetic control of transposon transcription and mobility in Arabidopsis.

E. Bucher, J. Reinders, M. Mirouze

Curr Opin Plant Biol (2012) 15:503–510.

 

Loss of DNA methylation affects the recombination landscape in Arabidopsis.

M.Mirouze, M. Lieberman-Lazarovich, R. Aversano, E. Bucher, J. Nicolet, J. Reinders and Jerzy Paszkowski

Proceedings of the National Academy of Sciences (2012).doi:10.1073/pnas.1120841109

 

An siRNA pathway prevents transgenerational retrotransposition in plants subjected to stress. 

H.Ito*, H. Gaubert*, E. Bucher*, M. Mirouze, I. Vaillant, J. Paszkowski

Nature (2011) 472:115–119.

*authors contributed equally

 

Stress-induced activation of heterochromatic transcription.

M. Tittel-Elmer, E. Bucher, L. Broger, O. Mathieu, J. Paszkowski, I. Vaillant

PLoS Genet (2010) vol. 6 (10) pp. e1001175

 

RNA-directed DNA methylation and plant development require an IWR1-type transcription factor.

T. Kanno*, E. Bucher*, L. Daxinger, B. Huettel, D. P. Kreil, F. Breinig, M. Lind, M. J. Schmitt, S. A. Simon, S. Guna Ranjan Gurazada, B. C. Meyers, Z. J. Lorkovic, A. J. M. Matzke, M. Matzke

EMBO reports, (2010). 11:65–71.

*authors contributed equally

 

MOM1 and Pol-IV/V interactions regulate the intensity and specificity of transcriptional gene silencing.

C. Yokthongwattana*, E. Bucher*1, M. Čaikovski, I. Vaillant, J. Nicolet, O. Mittelsten Scheid and J. Paszkowski

EMBO J. (2010); 29:340–351.

*authors contributed equally, 1Corresponding author

 

Selective epigenetic control of retrotransposition in Arabidopsis.

M. Mirouze, J. Reinders, E. Bucher, T. Nishimura, K. Schneeberger, S. Ossowski, J. Cao, D. Weigel, J. Paszkowski & O. Mathieu

Nature (2009) vol. 461 (7262) pp. 427-30

Compromised stability of DNA methylation and transposon immobilization in mosaic Arabidopsis epigenomes.

Reinders J., B.B.H. Wulff, M. Mirouze, A. Mari-Ordonez, M. Dapp, W. Rozhon, E. Bucher, G. Theiler, J. Paszkowski

Genes & Development (2009) vol. 23 (8) pp. 939-950

 

A stepwise pathway for biogenesis of 24-nt secondary siRNAs and spreading of DNA methylation. 

L. Daxinger, T. Kanno, E. Bucher, J. van der Winden, U. Naumann, A.J. Matzke and M. Matzke

EMBO J (2009) 28, 48-57.

 

A structural-maintenance-of-chromosomes hinge domain-containing protein is required for RNA-directed DNA methylation. 

T. Kanno*, E. Bucher*, L. Daxinger, B. Huettel, G. Bohmdorfer, W. Gregor, D.P. Kreil, M. Matzke and A.J. Matzke

Nat Genet (2008) 40:670-5.

*authors contributed equally

 

RNA-directed DNA methylation mediated by DRD1 and Pol IVb: a versatile pathway for transcriptional gene silencing in plants. 

B. Huettel, T. Kanno, L. Daxinger, E. Bucher, J. van der Winden, A.J. Matzke and M. Matzke

Biochim Biophys Acta (2007) 1769:358-74.

 

Multiple virus resistance at a high frequency using a single transgene construct. 

E. Bucher, D. Lohuis, P.M. van Poppel, C. Geerts-Dimitriadou, R. Goldbach and M. Prins

J Gen Virol (2006) 87:3697-701.

 

The influenza A virus NS1 protein binds small interfering RNAs and suppresses RNA silencing in plants. 

E. Bucher, H. Hemmes, P. de Haan, R. Goldbach, and M. Prins

J Gen Virol (2004) 85:983-91.

 

Resistance mechanisms to plant viruses: an overview. 

Goldbach, R., E. Bucher, and M. Prins.

Virus Res (2003) 92:207-12.

 

Negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positions. 

E. Bucher, T. Sijen, P. De Haan, R. Goldbach, and M. Prins

J Virol (2003) 77:1329-36.

by Etienne Bucher